최적의 발효 환경을 위한 요구 사항

Choosing The Right Pump

Bioprocess engineers need to consider the following factors while choosing the right pump:

Sensitivity of the fluid to shear-forces

Some fluids are very sensitive to shear-forces placed upon them, hence, the right pump should ensure a gentle pumping motion that does not lead to altering the fluid components (i.e. destroying cells in the fluid due to high shear-forces).

bioprocessing-fermentation_sensitivity-of-the-fluid-to-shear-forces

Need for sterility of the fluid path

Since a sterile environment is a crucial factor in determining whether aseptic fermentation is successful, contact of the organic matter with hardware components should be limited to only those parts that follow the highest sanitary standards.

bioprocessing-fermentation_need-for-sterility-of-the-fluid-path

Accuracy and Precision

Accurate and precise delivery of the organic fluid to the bioreactor is an important aspect of the fermentation process. The achievable accuracy and precision depend largely on the pump technology. In order to achieve as good accuracy and precision as possible, calibration and usage of sensors can help to overcome limitations inherent to certain pump technologies.

bioprocessing-fermentation_accuracy-and-precision

Why is the right pump crucial for Bioreactors?

The most important role that pumps play in fermentation is to regulate the many critical ingredients that go into a bioreactor. This includes measuring and controlling the pH-levels, feeding nutrients, measuring the pressure, and preventing foam formation.

bioprocessing-fermentation_why-is-pump-crucial

Nutrient Feed Pump (1)

During a bioprocess, microorganisms normally consume a wide range of nutrients, including water or highly viscous glucose solutions, which are delivered through the pump. In addition, it is necessary to provide various feed profiles so that pumps are able to ramp up fluid supply exponentially, which can be desired in a bioreactor.

Acid and Base Pumps (2,3)

Adding acid or base is sometimes necessary to maintain the desired environment within the bioreactor. This is usually indicated by detecting the pH level inside the device. The substances are added to the culture vessel through tubes and pumps, but their concentration needs to be skilfully attained.

Anti-foam Pump (4)

Foam is usually an undesired side effect of the fermentation process in bioreactors as it can block the flow of gas. In this case, pumps are used to control the formation of foam, and if necessary, pump in the anti-foam fluid to control foam.

Peristaltic Pumps – The Heart of Bioreactors

Considering the requirements in bioreactors, the ideal pump technology is peristaltic pumps. They stand out due to their ability to accurately and gently feed liquids, even with high viscosities, and to maintain the integrity of each fluid. Moreover, the fact that in peristaltic pumps tubes are the only components, that come in contact with the medium makes it easier to follow a high sanitary standard by using high quality tubing.

peristaltic pump ETL500

To learn more about peristaltic pump technology, visit our dedicated website and don’t forget to check out our new state-of-the-art peristaltic pump ETL500 which has been developed especially for transferring media in bioprocessing.